It is strongly recommended reading the detailed protocol sections of this user manual if the NucleoSpin® Gel and PCR Clean-up kit is used for the first time.. by Y Zhou · 2018 · Cited by 7 — Silica columns from PCR purification and gel extraction kits are widely used in ... In the laboratory, gene cloning can be performed using only manual ... DNA-​contaminated columns from a Qiagen gel extraction kit were .... Kit contents: Qiagen QIAquick Gel Extraction Kit, 250 rxn